Percorrer por autor "Dahlin, Christer"
A mostrar 1 - 3 de 3
Resultados por página
Opções de ordenação
- Consequences of early human saliva interaction on membranes for guided bone regenerationPublication . Kunrath, Marcel; Jolic, Martina; Giraldo-Osorno, Paula; Mendes, Karina; Rosa, Nuno; Dahlin, Christer
- Superhydrophilic nanotextured surfaces for dental implants: influence of early saliva contamination and wet storagePublication . Kunrath, Marcel F.; Correia, André; Teixeira, Eduardo R.; Hubler, Roberto; Dahlin, ChristerHydrophilic and nanotextured surfaces for dental implants have been reported as relevant properties for early osseointegration. However, these surface characteristics are quite sensitive to oral interactions. Therefore, this pilot study aimed to investigate the superficial alterations caused on hydrophilic nanotubular surfaces after early human saliva interaction. Titanium disks were treated using an anodization protocol followed by reactive plasma application in order to achieve nanotopography and hydrophilicity, additionally; surfaces were stored in normal atmospheric oxygen or wet conditioning. Following, samples were interacted with saliva for 10 min and analyzed regarding physical-chemical properties and cellular viability. Saliva interaction did not show any significant influence on morphological characteristics, roughness measurements and chemical composition; however, hydrophilicity was statistically altered compromising this feature when the samples were stored in common air. Cellular viability tested with pre-osteoblasts cell line (MC3T3-E1) reduced significantly at 48 h on the samples without wet storage after saliva contamination. The applied wet-storage methodology appears to be effective in maintaining properties such as hydrophilicity during saliva interaction. In conclusion, saliva contamination might impair important properties of hydrophilic nanotubular surfaces when not stored in wet conditions, suggesting the need of saliva-controlled sites for oral application of hydrophilic surfaces and/or the use of modified-package methods associated with their wet storage.
- Unveiling the consequences of early human saliva contamination on membranes for guided bone regenerationPublication . Kunrath, Marcel F.; Giraldo‐Osorno, Paula Milena; Mendes, Karina; Gomes, Ana T. P. C.; Rosa, Nuno; Barros, Marlene; Dahlin, ChristerAims: GBR membranes have various surface properties designed to elicit positive responses in regenerative clinical procedures; dental clinicians attempt to employ techniques to prevent the direct interaction of contaminated oral fluids with these biomaterials. However, saliva is uninterruptedly exhibited in oral surgical proce-dures applying GBR membranes, suggesting a persistent interaction with biomate-rials and the surrounding oral tissues. This fundamental study aimed to investigate potential alterations in the physical, chemical, and key biological properties of membranes for guided bone regeneration (GBR) caused by isolated early interac-tion with human saliva.Methods: A reproducible step- by- step protocol for collecting and interacting human saliva with membranes was developed. Subsequently, membranes were evaluated for their physicochemical properties, protein quantification, DNA, and 16S rRNA levels viability of two different cell lines at 1 and 7 days, and ALP activity. Non-interacted membranes and pure saliva of donors were applied as controls.Results: Qualitative morphological alterations were noticed; DNA extraction and 16S quantification revealed significantly higher values. Furthermore, the viability of HGF- 1 and MC3T3-E1 cells was significantly (p< .05) reduced following saliva inter-action with biodegradable membranes. Saliva contamination did not prejudice PTFE membranes significantly in any biological assay.Conclusions: These outcomes demonstrated a susceptible response of biodegrad-able membranes to isolated early human saliva interaction, suggesting impairment of structural morphology, reduced viability to HGF-1 and MC3T3-E1, and higher ab-sorption/adherence of DNA/16S rRNA. As a result, clinical oral procedures may need corresponding refinements.