Percorrer por autor "Chrisostomo, Daniela Alvim"
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- EGCG and taxifolin modulate secretory activity and expression of dentinogenesis markers in odontoblast-like cellsPublication . Duque, Cristiane; Rabelo, Rafaela Laruzo; Braga, Gabriela Pacheco de Almeida; Soares, Igor Paulino Mendes; Souza, Maria Eduarda de; Chrisostomo, Daniela Alvim; Hebling, Josimeri; Costa, Carlos Alberto de Souza; Prakki, Anuradha; Regasini, Luís OctavioOdontoblasts are cells specialized in dentin matrix deposition and the first line of defense when the dentin–pulp complex is injured by pathological processes, such as dental caries and trauma. Natural compounds, such as flavonoids, could be useful to stimulate odontoblast activity and reparative dentinogenesis in vital pulp therapies, especially in immature permanent teeth. This study evaluated the effect of flavonoids on odontoblast secretory activity and the expression of dentinogenesis markers. The effect of flavonoids was evaluated on phenotypic mineralization markers (alkaline phosphatase (ALP) activity and mineralized nodule deposition) by colorimetric assays and on the expression of Alpl, Mmp2, Mmp9, Dmp1, and Dspp genes in odontoblast-like cells by quantitative polymerase chain reaction. Most of the flavonoids did not show toxicity between 100 and 25 μM. In distinct concentrations, epigallocatechin gallate (EGCG), taxifolin, myricetin, quercetin, and kaempferol stimulated the activity of ALP and increased mineralized nodule deposition. However, the highest effect on those phenotypic markers was observed after EGCG and taxifolin treatments. Then, they were selected for evaluation of gene expression. mRNA levels of Dmp1 and Dspp highly increased with taxifolin treatment, and Alpl expression was increased for both taxifolin and EGCG groups, without difference between them. Mmp2 and Mmp9 expression was not affected by these flavonoids. In conclusion, EGCG and taxifolin positively affect phenotypic mineralization markers; in particular, taxifolin highly stimulates early- and late-stage dentinogenesis genes.
- Methacrylated epigallocatechin gallate functionalized dental adhesives: antiproteolytic activity and dentin bonding studiesPublication . Duque, Cristiane; Chrisostomo, Daniela Alvim; Scaffa, Polliana Mendes Candia; Gouveia, Zach; Nascimento, Fabio Dupart; Plotnikov, Sergey V.; Prakki, AnuradhaObjectives: To assess the antiproteolytic effect of EGCG-methacrylate monomers and its inhibitory effect on gelatinolytic activity in the hybrid layer. Also, to investigate the effect of an adhesive material functionalized with EGCG-methacrylate monomers on immediate and long-term dentin-resin bond strength. Methods: Neat EGCG (E0) was reacted with three different ratios of methacryloyl ester and dissolved in ethyl acetate to obtain EGCG-methacrylates with hydroxyl functionalization at 33 % (M-E33), 67 % (M-E67) and 100 % (M-E100) levels. Resin composite blocks were built on human dentin surfaces using self-etching adhesive containing E0, M-E33, M-E67, and M-E100 at 1 wt%. Demineralized human dentin disks were immersed in deionized water (DW) or lactic acid (LA) and subsequently treated with DW, acetone (as controls), E0, M-E33, M-E67 and M-E100 diluted in acetone. Concentrations of solubilized type I collagen C-terminal (CTX and ICTP) and N-terminal (NTX) telopeptides were determined from 7-day extracts of dentin matrix specimens by ELISA assays. In situ zymography of adhesive-dentin interface slices from restored teeth was performed by confocal microscope after 24 h dentin treatment. Microtensile bond strength (µTBS) and failure pattern were evaluated after 24 h and 6 months. Data were analyzed using two-way ANOVA and Tukey post hoc test (p < 0.05). Results: All experimental groups statistically reduced the release of solubilized telopeptides from dentin samples in DW and LA. E0 and M-E100 incorporated into the adhesive system reduced the gelatinolytic activity within the hybrid layer. The lowest µTBS values for restored teeth were observed for E0 and M-E100 groups, after 24 h and 6 months, respectively. The most prevalent failure observed was classified as type 4, except for M-E100. Significance: EGCG-methacrylate monomers effectively protected collagen from degradation. When incorporated into adhesive systems, EGCG-methacrylates reduced gelatinolytic activity within the hybrid layer, and did not affect immediate and long-term bond strength values of restorations.
- Root canal instrumentation of artificial primary teeth with rotary and reciprocating files: a micro-CT analysisPublication . Chrisostomo, Daniela Alvim; Danelon, Marcelle; Furlan, Renan Diego; Duarte, Marco Antônio Hungaro; Mello-Moura, Anna Carolina Volpi; Duque, CristianeThis study compared the outcomes of two endodontic instrumentation protocols (rotary or reciprocating files), using artificial primary teeth and micro-computed tomography. Twenty-four artificial primary molars were equally distributed into two groups of 12, according to the type of instrumentation – rotary files (Sequence Baby NiTi Files©) or reciprocating files (X1-Blue File NiTi files©). The following parameters were evaluated: root canal and dentin volumes, canal transportation and centering ability, risk of root perforation, and time of instrumentation. Statistically significant differences between the two instrumentation protocols considering root canal transportation and centering ability were identified. There was no difference in dentin thickness, fractures, and cracks comparing preoperative and postoperative time points for both endodontic files. Although the time of instrumentation was shorter for reciprocating files, rotary files promoted smaller root canal enlargement. Instrumentation with reciprocating and rotary files proved generally safe for pulpectomy in primary molars, promoting a negligible reduction in dentin volume, canal transportation, and centering ability, thereby preserving dentin thickness and lowering the risk of fractures.