Browsing by Author "Carvalho, Ana Paula"
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- Multi-step subcritical water extracts of fucus vesiculosus l. And codium tomentosum stackhouse: Composition, health-benefits and safetyPublication . Soares, Cristina; Paíga, Paula; Marques, Marta; Neto, Tânia; Carvalho, Ana Paula; Paiva, Alexandre; Simões, Pedro; Costa, Leonor; Bernardo, Ana; Fernández, Naiara; Pinto, Edgar; Almeida, Agostinho; Bronze, Maria Rosário; Delerue-Matos, Cristina; Grosso, ClaraMental health and active aging are two of the main concerns in the 21st century. To search for new neuroprotective compounds, extracts of Codium tomentosum Stackhouse and Fucus vesiculosus L. were obtained through multi-step (four step) subcritical water extraction using a temperature gradient. The safety assessment of the extracts was performed by screening pharmaceutical compounds and pesticides by UHPLC-MS/MS, and iodine and arsenic levels by ICP-MS. Although the extracts were free of pharmaceutical compounds and pesticides, the presence of arsenic and high iodine contents were found in the first two extraction steps. Thus, the health-benefits were only evaluated for the fractions obtained in steps 3 and 4 from the extraction process. These fractions were tested against five brain enzymes implicated in Alzheimer’s, Parkinson’s, and major depression etiology as well as against reactive oxygen and nitrogen species, having been observed a strong enzyme inhibition and radical scavenging activities for the step 4 fractions from both seaweed species. Regarding the variation of the chemical composition during the extraction, step 1 fractions were the richest in phenolic compounds. With the increase in temperature, Maillard reaction, caramelization and thermo-oxidation occurred, and the resulting products positively affected the antioxidant capacity and the neuroprotective effects.
- Nutritional characterization of acorn flour (a traditional component of the Mediterranean gastronomical folklore)Publication . Silva, Sara; Costa, Eduardo M.; Borges, André; Carvalho, Ana Paula; Monteiro, Maria João; Pintado, M. E.Abstract Nowadays, acorns, the fruits of Quercus trees, are mostly associated with animal feed. However they are part of the traditional gastronomical folklore of severalMediterranean countries. Though several uses can be mentioned, one of the most common uses is powdering the acorns in order to produce a flour that can substitute regular corn flour in the manufacture breads and cakes. An example of this is acorn bread, a typical Portuguese loaf.However, to the best of our knowledge, there is no information about the nutritional value of these flours. As such, the main goal of this work was to describe the nutritional value of acorn flours from two different subspecies of Quercus abundant in Portugal (Quercus ilex and Quercus rotundifolia) obtained using two different traditional methods (drying and roasting). The results demonstrated that all flours possessed interesting nutritional properties, namely the absence of gluten and elevated values of both monounsaturated and polyunsaturated fatty acids. Because of these facts and it’s low production cost, acorn flour poses as an interesting alternative to traditional flours particularly for gluten intolerant individuals.
- Spent yeast waste streams as a sustainable source of bioactive peptides for skin applicationsPublication . Costa, Eduardo M.; Oliveira, Ana Sofia; Silva, Sara; Ribeiro, Alessandra B.; Pereira, Carla F.; Ferreira, Carlos; Casanova, Francisca; Pereira, Joana O.; Freixo, Ricardo; Pintado, Manuela E.; Carvalho, Ana Paula; Ramos, Óscar L.Spent yeast waste streams are a byproduct obtained from fermentation process and have been shown to be a rich secondary source of bioactive compounds such as phenolic compounds and peptides. The latter are of particular interest for skin care and cosmetics as they have been shown to be safe and hypoallergenic while simultaneously being able to exert various effects upon the epidermis modulating immune response and targeting skin metabolites, such as collagen production. As the potential of spent yeast’s peptides has been mainly explored for food-related applications, this work sought to understand if peptide fractions previously extracted from fermentation engineered spent yeast (Saccharomyces cerevisiae) waste streams possess biological potential for skin-related applications. To that end, cytotoxic effects on HaCat and HDFa cells and whether they were capable of exerting a positive effect upon the production of skin metabolites relevant for skin health, such as collagen, hyaluronic acid, fibronectin and elastin, were evaluated. The results showed that the peptide fractions assayed were not cytotoxic up to the highest concentration tested (500 µg/mL) for both cell lines tested. Furthermore, all peptide fractions showed a capacity to modulate the various target metabolites production with an overall positive effect being observed for the four fractions over the six selected targets (pro-collagen IαI, hyaluronic acid, fibronectin, cytokeratin-14, elastin, and aquaporin-9). Concerning the evaluated fractions, the overall best performance (Gpep > 1 kDa) was of an average promotion of 41.25% over the six metabolites and two cell lines assessed at a concentration of 100 µg/mL. These results showed that the peptide fractions assayed in this work have potential for future applications in skin-related products at relatively low concentrations, thus providing an alternative solution for one of the fermentation industry’s waste streams and creating a novel and highly valuable bioactive ingredient with encompassing activity to be applied in future skin care formulations.