Browsing by Author "Baptista-Silva, S."
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- Biomechanical performance of hybrid electrospun structures for skin regenerationPublication . Dias, J. R.; Baptista-Silva, S.; Sousa, A.; Oliveira, A. L.; Bártolo, P. J.; Granja, P. L.Wound dressings made by electrospun nanofibers have been demonstrating great potential to regenerate skin tissue as compared to the conventional membrane products available in the market. Until today most of the developed dressings have only demonstrated the capability to regenerate the dermis or epidermis. In this study we propose new hybrid electrospun meshes combining polycaprolactone and gelatin. Several approaches, multilayer, coating and blend were stablished to investigate the most appropriate hybrid structure with potential to promote skin regeneration in its full thickness. The structures were evaluated in terms of physico-chemical properties (porosity, water vapor permeability, contact angle and swelling degree) and according to its mechanical and biological performance. Multilayer and blend structures demonstrated to fit most of native skin requirements. However, looking to all the performed characterization we considered multilayer as the most promising hybrid structures, due its high porosity which contributed to an ideal water vapor permeability rate and good mechanical and biological properties. Based on this multilayer structure is a promisor wound dressing.
- In situ antioxidant activity of an enzymatically crosslinked sericin hydrogel for healing of chronic woundsPublication . Bernardes, B. G.; Baptista-Silva, S.; Borges, S.; Pintado, M.; Granja, P. L.; Soares, R.; Costa, R.; Oliveira, A. L.
- In situ antioxidant activity of an enzymatically crosslinked sericin hydrogel towards chronic wound healingPublication . Bernardes, B. G.; Baptista-Silva, S.; Borges, S.; Pintado, M.; Granja, P. L.; Soares, R.; Costa, R.; Oliveira, A. L.
- In situ crosslinked electrospun gelatin nanofibers for skin regenerationPublication . Dias, J. R.; Baptista-Silva, S.; Oliveira, C. M. T. de; Sousa, A.; Oliveira, Ana L.; Bártolo, P. J.; Granja, P. L.Due to its intrinsic similarity to the extracellular matrix, gelatin electrospun nanofibrous meshes are promising scaffold structures for wound dressings and tissue engineering applications. However, gelatin is water soluble and presents poor mechanical properties, which generally constitute relevant limitations to its applicability. In this work, gelatin was in situ crosslinked with 1,4-butanediol diglycidyl ether (BDDGE) at different concentrations (2, 4 and 6 wt%) and incubation time-points (24, 48 and 72 h) at 37 °C. The physico-chemical and biological properties of BDDGE-crosslinked electrospun gelatin meshes were investigated. Results show that by changing the BDDGE concentration it is possible to produce nanofibers crosslinked in situ with well-defined morphology and modulate fiber size and mechanical properties. Crosslinked gelatin meshes show no toxicity towards fibroblasts, stimulating their adhesion, proliferation and synthesis of new extracellular matrix, thereby indicating the potential of this strategy for skin tissue engineering.
- In-situ forming silk sericin-based hydrogel for chronic wound healingPublication . Baptista-Silva, S.; Borges, S.; Costa-Pinto, A. R.; Ramos, O. S.; Alves, P.; Granja, P. L.; Oliveira, A. L.
- A view on polymer-based composite materials for smart wound dressingsPublication . Baptista-Silva, S.; Alves, P.; Guimarães, I.; Borges, S.; Tavaria, F.; Granja, P.; Pintado, M.; Oliveira, A. L.Wound management challenges everyday thousands of health professionals, mainly due to the constant monitoring and difficulties in deciding the correct treatment options. When considering chronic wounds, selecting the ideal dressing defies clinical knowledge, when facing the large amount of different materials, its distinctive properties and the uniqueness of each patient needs. This chapter presents an overview on the challenges and complexity of a chronic wound, exploring the event of awound infection and discussing the large range of polymer-based composite materials and products in use for each specific wound condition, taking into account the key decision aspects defined by the clinicians. Different tissue engineering strategies are also herein addressed with varied reported clinical success, ranging from non-cellularized to considerably sophisticated cellularized products, reproducing the compositional complexity of both dermis and epidermis. Recent advances in smart dressings and sensors are also brought to discussion as sensing the wound can give us new insights about the series of complex biochemical events related to the healing and regeneration process, while contributing for a better wound assessment.