Maia, Alexandra S.Ribeiro, Ana R.Amorim, Catarina L.Barreiro, Juliana C.Cass, Quezia B.Castro, Paula M.L.Tiritan, Maria E.2013-11-142013-11-142013-06MAIA, Alexandra S. [et al.] - Enantioselective Determination of Fluoxetine and Norfluoxetine in Wastewater. In 39th International Symposium on High Performance Liquid Phase Separations and Related Techniques, Amsterdam, The Netherlands, 16-20 June 2013 – In HPLC 2013 - 39th International Symposium on High Performance Liquid Phase Separations and Related Techniques: Book of Abstracts, (2013) p.379http://hdl.handle.net/10400.14/13242Antibiotics are a therapeutic class widely found inenvironmental matrices and extensively studied due to its persistence and implications for multi-resistant bacteria development. Degradation of four fluoroquinolone antibiotics, namely Ofloxacin (OFL), Norfloxacin (NOR), Ciprofloxacin (CPF) and Moxifloxacin (MOX), at 10 mg L-1 using a mixed bacterial culture, was assessed for 60 days. The assays were followed by a developed and validated analytical method of HPLC with Fluorescence Detection using a Luna PFP (2) 3µm column. The optimized conditions allowed picturing metabolites/transformation products formation and accumulation during the process, stating an incomplete mineralization, also shown byfluoride release. OFL and MOX presented the highest (98.3%) and the lowest (80.5%) extent of degradation after 19 days of assay, respectively. Some of these intermediate compounds were identified by LCMS/MS in selected degradation samples. Most of the intermediates were already described as biodegradation and/or photodegradationproducts in different conditions, but new and/or unknown metabolites were also present.engBiodegradationFluoroquinolonesLC-MS/MSMetabolitesconference object