Casquete, RocíoFonseca, Susana C.Pinto, RicardoCastro, Sónia M.Todorov, SvetoslavTeixeira, PaulaVaz-Velho, Manuela2019-10-172019-10-172019Casquete, R., Fonseca, S. C., Pinto, R., Castro, S. M., Todorov, S., Teixeira, P., & Vaz-Velho, M. (2019). Evaluation of the microbiological safety and sensory quality of a sliced cured-smoked pork product with protective cultures addition and modified atmosphere packaging. Food Science and Technology International, 25(4), 327–336. https://doi.org/10.1177/10820132198257711082-0132http://hdl.handle.net/10400.14/28414The aim of this study was to evaluate the effect of two protective lactic acid bacteria cultures combined with modified atmosphere packaging on the survival/growth of Listeria innocua 2030c (as a surrogate for Listeria monocytogenes) and on sensory attributes of ready-to-eat ‘lombo’ over storage time. Sliced ‘lombo’, a traditional cured-smoked pork loin, was inoculated with L. innocua 2030c, Lactobacillus sakei ST153 (isolated from ‘salpicão’) and BLC35 culture (with Lactobacillus curvatus, Staphylococcus xylosus and Pediococcus acidilactici; CHR Hansen) as protective cultures. Samples were packed in two modified atmosphere packaging conditions (20% CO2/80% N2 and 40% CO2/60% N2) and stored at 5 ℃ for 124 days. Both cultures led to a reduction of 1–2 log CFU/g of L. innocua 2030c after 12 h; however, at the end of storage only Lb. sakei ST153 maintained this antilisterial effect, which was more evident at 40% CO2/60% N2. The influence of cultures addition and modified atmosphere packaging conditions on the sensory characteristics of the product were not significant. Thus, Lb. sakei ST153 combined with modified atmosphere packaging is a strong candidate to be used in a biopreservation strategy maintaining the traditional sensory quality of cured-smoked pork products and increasing their safety with respect to Listeria spp.engMeat productBacteriocinsLactic acid bacteriaModified atmosphere packagingSensory propertiesListeria monocytogenesjournal article10.1177/10820132198257711532-17388506073674130669863000469359000006