Vaz-Moreira, IvoneFigueira, VâniaLopes, Ana R.Lobo-da-Cunha, AlexandreSproer, CathrinSchumann, PeterNunes, Olga C.Manaia, Célia M.2013-01-282013-01-282012VAZ-MOREIRA, Ivone ...[et al.] - Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant. International Journal of Systematic and Evolutionary Microbiology. ISSN 1466-5034. Vol. 62, n.º 1 (2012), p. 71–771466-5026http://hdl.handle.net/10400.14/9994A Gram-positive, aerobic, non-motile, endospore-forming rod, designated DS22T, was isolated from a drinking-water treatment plant. Cells were catalase- and oxidase-positive. Growth occurred at 15–37 6C, at pH 7–10 and with ,8% (w/v) NaCl (optimum growth: 30 6C, pH 7–8 and 1–3% NaCl). The major respiratory quinone was menaquinone 7, the G+C content of the genomic DNA was 36.5 mol% and the cell wall contained meso-diaminopimelic acid. On the basis of 16S rRNA gene sequence analysis, strain DS22T was a member of the genus Bacillus. Its closest phylogenetic neighbours were Bacillus horneckiae NRRL B-59162T (98.5% 16S rRNA gene sequence similarity), Bacillus oceanisediminis H2T (97.9 %), Bacillus infantis SMC 4352-1T (97.4 %), Bacillus firmus IAM 12464T (96.8 %) and Bacillus muralis LMG 20238T (96.8 %). DNA–DNA hybridization, and biochemical and physiological characterization allowed the differentiation of strain DS22T from its closest phylogenetic neighbours. The data supports the proposal of a novel species, Bacillus purgationiresistans sp. nov.; the type strain is DS22T (5DSM 23494T5NRRL B-59432T5LMG 25783T).engjournal article10.1099/ijs.0.028605-01466-5034WOS:000300136200013