Coscueta, EzequielPintado, Maria Manuela2026-06-092026-06-092022-08-1760d09b67-9ba4-4b2d-879e-e92b4a11f1a9http://hdl.handle.net/10400.14/58056This protocol describes the procedure for the determination of the IC50 in inhibition on angiotensin-I converting enzyme (ACE) activity. ACE is also known as peptidyl dipeptidase A because it removes C-terminal dipeptides from a wide variety of peptide substrates. In the assay described here, the chosen substrate is the intramolecularly quenched fluorescent tripeptide o-aminobenzoylglycyl-p-nitrophenylalanylproline (Abz–Gly–Phe(NO2)–Pro). Hydrolysis of this substrate by the action of ACE generates the fluorescent product o-aminobenzoylglycine (Abz–Gly).engACEAngiotensin-converting enzymeAntihypertensiveIC50BioactivityAngiotensin-l converting enzymeAngiotensinInhibitory activity assayIc50 in inhibitionNitrophenylalanylprolineEnzymeTerminal dipeptidePeptide substrateAction of aceConverting enzymeAssayother10.17504/protocols.io.q26g74q5kgwz/v1