Teixeira, FilipaSilva, Ana MargaridaSut, StefaniaDall'Acqua, StefanoRamos, Oscar L.Ribeiro, Alessandra B.Ferraz, RicardoDelerue-Matos, CristinaRodrigues, Francisca2024-05-222024-05-222024-070963-9969http://hdl.handle.net/10400.14/45238Lycium barbarum L. berries have a remarkable chemical composition and extensive biological activities, being a valuable component of health and nutraceutical practices. Nevertheless, a deep insight on the intestinal permeation of the pro-healthy bioactive compounds is urgently needed to predict the real effects on human body. This study attempted, for the first time, to optimize the Ultrasound-Assisted Extraction (UAE) of goji berries using a Response Surface Methodology approach and establish the intestinal permeation of the principal pro-healthy compounds. The optimal extraction conditions were a solid:liquid ratio of 8.75 % for 56.21 min, using an intensity of 59.05 W/m2. The optimal extract displayed a remarkable antioxidant capacity, with LC/DAD-ESI-MS analysis unveiled a diverse phytochemical profile, encompassing different compounds (e.g. glu-lycibarbarspermidine F, 2-glu-kukoamine, rutin, 3,5-dicaffeoylquinic acid). The intestinal co-culture model demonstrated that glu-lycibarbarspermidine F (isomer 2) (73.70 %), 3,5-dicaffeoylquinic acid (52.66 %), and isorhamnetin-3-O-rutinoside (49.31 %) traversed the intestinal cell layer, exerting beneficial health-promoting effects.engIntestinal permeationLycium barbarumPhenolic compoundsResponse surface methodologyUltrasound-assisted extractionjournal article10.1016/j.foodres.2024.1145028519283109738823845001241464900001