Fernandes, A.Lopes, A.Magalhães, R.Oliveira, C.Pintado, M.Tavaria, F.2025-10-202025-10-202025-07-01Fernandes, A., Lopes, A., Magalhães, R., & Oliveira, C. et al. (2025). Mushroom-derived polysaccharides as bioactive agents for skin regeneration: evaluation of antimicrobial, wound-healing, and immunomodulatory effects. FEBS Open Bio, 15(S2), 207-207. Article P-28-067.2211-5463c54af103-151d-4f4b-9ccb-b201da21c623http://hdl.handle.net/10400.14/55400Mushroom polysaccharides have emerged as promising bioactive agents for enhancing skin regeneration. This study evaluates three polysaccharide-rich fractions (SB, SC, RD) obtained by hot water and alkaline extractions from Coriolus versicolor, Pleurotus ostreatus, and Hericium erinaceus. Their potential to promote skin repair was investigated by analyzing effects on cell proliferation and migration, immune modulation, and microbial growth inhibition. These fractions were previously characterized by several chemical and structural characterization methods that revealed significant presence of bioactive molecules (e.g., glucans, proteins, phenolics). Cell viability was accessed by PrestoBlue and MTT assays in HaCat and L929 cell lines, revealing the non-toxicity of the compounds at tested concentrations. Furthermore, proliferation assays (BrdU incorporation) and migration assays (scratch assay) in HaCaT cells were conducted at optimized concentrations (0.6 and 0.3 mg/mL). Notably, C. versicolor fractions promoted wound closure of the injured monolayer by 95% after 48 h (at 0.6 mg/mL) compared to 66% in the non-treated control. The fractions exhibited broadspectrum antimicrobial and antibiofilm activity in Gram-positive bacteria (S. epidermidis, MRSA), Gram-negative bacteria (P. aeruginosa, E. coli) and yeast (C. albicans, C. tropicalis). Microbial growth inhibition varied across the three species and extraction method, ranging from 30% to 70% at a concentration of 20 mg/mL. Additionally, immunomodulatory effects are being assessed by measuring key pro-inflammatory cytokines (TNF-?, IL-17A, IL-1?, IL-6) and anti-inflammatory (IL-10) to elucidate how these polysaccharides regulate inflammation during healing and skin repair. These findings highlight the potential of polysaccharides as multifunctional agents in dermatological applications, supporting their role in developing innovative, natural-based therapies for skin regeneration.engconference object001552034502188