Acetylated cashew gum and fucan for incorporation of lycopene rich extract from red guava (Psidium guajava L.) in nanostructured systems: antioxidant and antitumor capacity

Andrades, Eryka Oliveira deCosta, João Marcos Antônio Rodrigues daNeto, Francisco Edmar Moreira de LimaAraujo, Alyne Rodrigues deRibeiro, Fabio de Oliveira SilvaVasconcelos, Andreanne GomesOliveira, Antônia Carla de JesusSobrinho, José Lamartine SoaresAlmeida, Miguel Peixoto deCarvalho, Ana P.Dias, Jhones NascimentoSilva, Ingrid Gracielle MartinsAlbuquerque, PatríciaPereira, Ildinete SilvaRabello, Doralina do AmaralAmorim, Adriany das Graças NascimentoLeite, José Roberto de Souza de AlmeidaSilva, Durcilene Alves da2021-10-202022-10-022021-11-300141-8130http://hdl.handle.net/10400.14/35630Industrial application of lycopene is limited due to its chemical instability and low bioavailability. This study proposes the development of fucan-coated acetylated cashew gum nanoparticles (NFGa) and acetylated cashew gum nanoparticles (NGa) for incorporation of the lycopene-rich extract from red guava (LEG). Size, polydispersity, zeta potential, nanoparticles concentration, encapsulation efficiency, transmission electron microscopy (TEM) and atomic force microscopy (AFM) were used to characterize nanoparticles. The antioxidant activity was determinated and cell viability was evaluated in the human breast cancer cells (MCF-7) and human keratinocytes (HaCaT) by MTT assay. The toxic effect was evaluated by hemolysis test and by Galleria mellonella model. NFGa showed higher stability than NGa, having a size of 162.10 ± 3.21 nm, polydispersity of 0.348 ± 0.019, zeta potential -30.70 ± 0.53 mV, concentration of 6.4 × 109 nanoparticles/mL and 60% LEG encapsulation. Microscopic analysis revealed a spherical and smooth shape of NFGa. NFGa showed antioxidant capacity by ABTS method and ORAC assay. The NFGa presented significant cytotoxicity against MCF-7 from the lowest concentration tested (6.25-200 μg/mL) and did not affect the cell viability of the HaCaT. NFGa showed non-toxic effect in the in vitro and in vivo models. Therefore, NFGa may have a promising application in LEG stabilization for antioxidant and antitumor purposes.engAntioxidantAntitumorBiocompatibilityLycopeneNanoparticlesPolysaccharidesAcetylated cashew gum and fucan for incorporation of lycopene rich extract from red guava (Psidium guajava L.) in nanostructured systems: antioxidant and antitumor capacityjournal article10.1016/j.ijbiomac.2021.09.1168511613397734563578000710232400003