Pais, Teresa F. M.Vidigal, Susana S. M. P.Tóth, Ildikó V.Rangel, António O. S. S.2013-01-282013-01-282013PAIS, Teresa F. M... [et al.] - Sequential injection system for the enzymatic determination of ethanol in alcoholic beverages with in-line dilution. Food Control. ISSN 0956-7135. Vol. 30, n.º 2 (2013), p. 616-6200956-7135http://hdl.handle.net/10400.14/9986A sequential injection system for the enzymatic determination of ethanol in wines with spectrophotometric detection was developed. The determination was based on the enzymatic reaction involving ethanol and NAD(+) in the presence of alcohol dehydrogenase. A gas-diffusion unit was introduced in the system to perform an in-line dilution. It was possible to operate the system with a reagent consumption of 1.3 mg of NAD(+), 0.45 U of ADH, and 32 mu L of sample, per assay with a determination rate of 21 h(-1). The results obtained from the analysis of wine samples were statistically equivalent to those obtained by the reference method, with good repeatability; the relative standard deviation (n = 10) was <1% with a LOQ of 0.3% (v/v). (C) 2012 Elsevier Ltd. All rights reserved.engSequential injection analysis systemEthanol determinationWineAlcohol dehydrogenaseGas-diffusion unitSpectrophotometryjournal article10.1016/j.foodcont.2012.08.0131873-7129WOS:000313535500042