Bergera, Lúcia Raquel RamosStamford, Thayza Christina MontenegroOliveira, Kataryne Árabe RimádePessoa, Adjanede Miranda PereiraLima, Marcos Antonio Barbosa dePintado, Maria Manuela EstevezCâmara, Marcos Paz SaraivaFranco, Lucianade OliveiraSouza, Marciane Magnania Evandro Leite deSouza, Evandro Leite de2018-11-052018-11-052018Berger, L.R.R., Stamford, T.C.M., Oliveira, K.Á.R., Pessoa, A.M.P., Lima, M.A.B., Pintado, M.E., … Souza, E.L. (2018). Chitosan produced from Mucorales fungi using agroindustrial by-products and its efficacy to inhibit Colletotrichum species. International Journal of Biological Macromolecules, 108, 635-6410141-8130http://hdl.handle.net/10400.14/25950This study evaluated corn steep liquor (CSL) and papaya peel juice (PPJ) in mixture as substrates for thecultivation (96 h, 28◦C, pH 5.6, 150 rpm) of Mucorales fungi for chitosan production, and determinedthe growth-inhibitory effect of the fungal chitosan (FuCS) obtained under optimized conditions againstphytopathogenic Colletotrichum species. All Mucorales fungi tested were capable of growing in CSL-PPJmedium, showing FuCS production in the range of 5.02 (Fennelomyces heterothalicus SIS 28) − 15.63 mg/g(Cunninghamella elegans SIS 41). Highest FuCS production (37.25 mg/g) was achieved when C. eleganswas cultivated in medium containing 9.43% CSL and 42.5% PPJ. FuCS obtained under these conditionsshowed a deacetylation degree of 86%, viscosity of 120 cP and molecular weight of 4.08 × 104g/mol.FuCS at 5000, 7500 and 10,000 ppm inhibited the growth of all Colletotrichum species tested. FuCS alsoinduced alterations in the morphology of C. fructicola hyphae. CSL-PPJ mixtures are suitable substratesfor the cultivation of Mucorales fungi for FuCS production. Chitosan from C. elegans cultivated in CSL-PPJmedium is effective in inhibiting phytopathogenic Colletotrichum species.engAgroindustrial by-productsFungal chitosanAntifungal effectsjournal article10.1016/j.ijbiomac.2017.11.1781879-00038503785849229199126000425071500075