Aguiar, Juliana I. S.Ribeiro, Susana O.Leite, AndreiaRangel, MariaRangel, António O. S. S.Mesquita, Raquel B. R.2026-01-302026-01-302022-06-01Aguiar, J. I. S., Ribeiro, S. O., Leite, A., & Rangel, M. et al. (2022). Development of a microfluidic paper-based analytical device (µPAD) for iron determination in water. In J. Kozak, A. Telk, A. Wo?niakiewicz, & J. Paluch (Eds.), XV Flow Analysis: book of abstracts (pp. 121-121). Jagiellonian University in Krakow.978839635041163bf6340-e925-4efd-8226-67b936fc551dhttp://hdl.handle.net/10400.14/56823Ingestion of waters with high iron concentration can cause several risks to human health. In this work, a new microfluidic paper-based analytical device (µPAD) was developed to quantify iron in surface and waste waters using a new fluorescent 3-hydroxy-4-pyridione ligand. Under optimal conditions, a linear calibration curve was obtained in the concentration range of 0.25 – 2.00 mg/L with a limit detection of 0.11 mg/L. The proposed µPAD method was successfully applied to the determination of iron in surface and waste water samples with no need for sample pre-treatment, and its accuracy evaluated by comparing to the AAS standard method (RD < 10%). The developed µPAD is simple, economical, sensitive and present low analysis time. Each µPAD uses low amounts of reagents, approximately 0.5 mg of ligand and 17 mg of sodium hydrogenocarbonate and sample consumption of 100 µL.engconference proceedings