Fontes, Ana LuizaPimentel, LígiaSoares, Ana Maria SilvaDomingues, M. RosárioRodríguez-Alcalá, Luis MiguelGomes, Ana Maria2023-02-142023-02-142023-07-010308-8146http://hdl.handle.net/10400.14/40209This work studied the viability of using vegetable oils as precursor substrates to develop a dairy product enriched in microbial conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids. Hydrolysis of hempseed, flaxseed (FSO) and soybean (SBO) oils was tested with Candida rugosa (CRL), Pseudomonas fluorescens, or Pancreatic porcine lipases. FSO and SBO, previously hydrolyzed with CRL, were further selected for cow’s milk CLA/CLNA-enrichment with Bifidobacterium breve DSM 20091. Thereafter, higher substrate concentrations with hydrolyzed FSO were tested. For all tested oils, CRL revealed the best degrees of hydrolysis (>90%). Highest microbial CLA/CLNA yield in milk was achieved with hydrolyzed FSO, which led to the appearance of mainly CLNA isomers (0.34 mg/g). At higher substrate concentrations, maximum yield was 0.88 mg/g CLNA. Therefore, it was possible to enrich milk with microbial CLNA using vegetable oil, but not with CLA, nor develop a functional product that can deliver a reliable effective dose.engConjugated linoleic acid (CLA)Conjugated linolenic acid (CLNA)Vegetable oilsLipasesMilkBifidobacterium brevejournal article10.1016/j.foodchem.2023.13566585147857701000946315100001