Rocha, JoanaBotelho, JoãoKsiezarek, MagdalenaUgarcina Perovic, SvetlanaMachado, MiguelCarrico, João AndréPimentel, Lígia L.Salsinha, SofiaRodríguez-Alcalá, Luis M.Pintado, ManuelaRibeiro, Teresa G.Peixe, Luísa2020-04-172020-04-172020Rocha, J., Botelho, J., Ksiezarek, M., Perovic, S. U., Machado, M., Carriço, J. A., ... Ribeiro, T. G. (2020). Lactobacillus mulieris sp. nov., a new species of Lactobacillus delbrueckii group. International Journal of Systematic and Evolutionary Microbiology, 70(3), 1522-15271466-5026http://hdl.handle.net/10400.14/30280One Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, and coccobacilli-shaped strain, designated c10Ua161MT, was isolated from a urine sample from a reproductive-age healthy woman. Comparative 16S rRNA gene sequence analysis indicated that strain c10Ua161MT belonged to the genus Lactobacillus . Phylogenetic analysis based on pheS and rpoA gene sequences strongly supported a clade encompassing strains c10Ua161MT and eight other strains from public databases, distinct from currently recognized species of the genus Lactobacillus. In silico Average Nucleotide Identity (ANI) and Genome-to-Genome Distance Calculator (GGDC), showed 87.9 and 34.3 % identity to the closest relative Lactobacillus jensenii , respectively. The major fatty acids of strain c10Ua161MT were C18 : 1ω9c (65.0%), C16 : 0 (17.8%), and summed feature 8 (10.2 %; comprising C18 : 1ω7c, and/or C18 : 1ω6c). The DNA G+C content of the strains is 34.2 mol%. On the basis of data presented here, strain c10Ua161MT represents a novel species of the genus Lactobacillus , for which the name Lactobacillus mulieris sp. nov. is proposed. The type strain is c10Ua161MT (=CECT 9755T=DSM 108704T).eng16S rRNApheSrpoAGenomeFatty acidjournal article10.1099/ijsem.0.0039011466-50348508278003331951193000522929700011