Silva, Sara Baptista daOliveira, AnaFerreira, DomingosSarmento, BrunoPintado, Manuela2014-05-152014-05-152013SILVA, S… [et al.] - Development and Validation Method for Simultaneous Quantification of Phenolic Compounds in Natural Extracts and Nanosystems. Phytochemical Analysis. ISSN 1099-1565. Vol. 24, N.º 6 (2013), p. 638-6440958-0344http://hdl.handle.net/10400.14/14431IntroductionSage and savoury (Salvia sp. and Satureja montana, respectively) are plants used in traditional medicine. The quality control of their herbal formulations is of paramount concern to guarantee the expected biological activity of their anti-oxidant compounds. Objective To establish a simple and effective high-performance liquid chromatographic (HPLC) method to evaluate simultaneously quercetin and rosmarinic acid, in a pure form, in natural extracts (sage and savoury), and encapsulated into chitosan nanoparticles. Methods Chromatography was performed on an RP C-18-column, in a gradient mode with a mobile phase comprising methanol:formic acid:water 92.5:2.5:5 (v/v) at a flow rate of 0.75mL/min and at wavelength of 280nm. Results The method was specific, linear in the range of 0.05-1mg/mL (R-2=1.00), precise at the intraday and interday levels, accurate (recovery rate 90.50.6%), and robust to changes in equipment conditions. Conclusion The method established was effective for quercetin and rosmarinic acid characterisation in natural extracts and in chitosan nanoparticles, allowing the loading capacity determination, the association efficiency as well as the in vitro release. This study shows a new HPLC method validated to simultaneous quantification of rosmarinic acid and quercetin in natural extracts and chitosan nanoparticles. This method is also able for detection and quantification of other phenolic and flavanoid compounds in natural matrix.engHPLCChitosan nanoparticlesQuercetinRosmarinic acidjournal article10.1002/pca.24461099-1565WOS:000326032100017