Silva, SaraCosta, Eduardo M.Machado, Manuela2026-04-092026-04-092026-03-30Silva, S., Costa, E. M., & Machado, M. (2026). Metabolite profiling of post-biotics obtained from fish by-products. 1-1. Poster session presented at ANALÍTICA 2026 - 12th Meeting of the SPQ Analytical Chemistry Division.d1489eb1-5fbf-499e-a2a5-7d5c53c22af1http://hdl.handle.net/10400.14/57476The FERDINAND project aims to valorize fish by-products as a sustainable source of bioactive compounds for applications in cardiometabolic health, focusing on the development and characterization of innovative post-biotic fractions. Post-biotic metabolite analysis provides critical insight into the biochemical transformations occurring during microbial fermentation and supports the valorization of fish by-products into high-value functional ingredients. In this study, fish-derived biomass is fermented using selected microbial strains, generating a complex mixture of post-biotics formed during the breakdown of proteins, lipids, and residual organic matter. A comprehensive analytical framework combining chromatographic and mass spectrometry-based techniques is applied to characterize the resulting metabolites, including short-chain fatty acids, organic acids, and peptide fragments. Targeted quantification is performed using GC-FID and HPLC-RI, while LC-MS/MS and untargeted metabolomics enable in-depth profiling of low-molecular-weight compounds. Integrating targeted and untargeted data provides a holistic understanding of post-biotic composition, facilitating the identification of safe, stable, and bioactive fractions derived from fish by-products with potential applications in food, nutraceutical, and biomedical sectors.engconference poster not in proceedings10.34632/d1489eb1-5fbf-499e-a2a5-7d5c53c22af1