Carvalho, Maria F.Marco, Paolo DeDuque, Anouk F.Pacheco, Catarina C.Janssen, Dick B.Castro, Paula M. L.2010-10-112010-10-112008"International Journal of Systematic and Evolutionary Microbiology". ISSN 1466-5026. 58: 3 (2008) 692–698http://hdl.handle.net/10400.14/2786A detailed classification of a novel bacterial strain, designated F11T, capable of degrading fluorobenzene as a sole carbon and energy source, was performed by using a polyphasic approach. This Gram-negative, rod-shaped, non-motile, non-spore-forming, aerobic bacterium was isolated from a sediment sample collected from an industrially contaminated site in northern Portugal. The predominant whole-cell fatty acids were C19 : 0 cyclo ω8c, C16 : 01 C18 : 1ω7c, C18 : 0,C18 : 0 3-OH and C16 : 0 3-OH. The G+C content of the DNA was 62.9 mol% and the major respiratory quinone was ubiquinone 10 (UQ-10). 16S rRNA gene sequence analysis revealed that strain F11T was a member of the class Alphaproteobacteria and was phylogenetically related to the genus Labrys, having sequence similarities of 95.6 and 93.1% to the type strains of Labrys monachus and Labrys methylaminiphilus, respectively. DNA–DNA hybridization experiments revealed levels of relatedness of ,70% between strain F11T and the type strains of L. monachus and L. methylaminiphilus (38.6 and 34.1 %, respectively), justifying the classification of strain F11T as representing a novel species of the genus Labrys. The name Labrys portucalensis sp. nov. is proposed for this organism. The type strain is F11T (5LMG 23412T5DSM 17916T).engjournal article10.1099/ijs.0.65472-0