Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/5484
Título: Carbodiimide modification enhances activity of pig pancreatic phospholipase A2
Autor: Ferreira, João Paulo M.
Sasisekharan, Ram
Louie, Otway
Langer, Robert
Data: 1994
Editora: Wiley Blackwell
Citação: FERREIRA, João Paulo M... [et al.] - Carbodiimide modification enhances activity of pig pancreatic phospholipase A2. European Journal of Biochemistry. ISSN 1742-4658. Vol. 223, n.º 2 (1994), p. 611–616
Resumo: Pig phospholipase A2, pig iso-phospholipase A2 and bovine pancreatic phospholipase A2 were reacted in solution with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, in the presence of N-hydroxysulfosuccinimide, at pH 7. The influence of micellar protectants was analyzed. In the presence of n-hexadecylphosphocholine, the losses of activity in micellar diheptanoyl-lecithin were 80, 35, and 10% in bovine phospholipase A2, pig iso-phospholipase A2, and pig phospholipase A2, respectively. With 1-oleoylglycerophosphocholine, the bovine enzyme lost 40% activity, but the pig enzyme was activated sevenfold. The modified pig enzyme showed pre-micellar activation on monomeric diheptanoyl-lecithin, and either reduced or increased activities on mixed micelles of bile salt with egg phosphatidylcholine, depending on the composition of the micelles. This activation is consistent with previous protein-engineering studies of pig pancreatic phospholipase A2. In this study, we present new information concerning the specificity and interfacial recognition behaviour of this enzyme in relation to this activation.
Peer review: yes
URI: http://hdl.handle.net/10400.14/5484
Versão do Editor: The definitive version is available at: http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1994.tb19032.x/pdf
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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