Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/18850
Título: In vitro ACE-inhibitory peptide KGYGGVSLPEW facilitates noradrenaline release from sympathetic nerve terminals:Relationship with the lack of antihypertensive effect on spontaneous hypertensive rats
Autor: Marques, Cláudia
Amorim, Maria Manuela
Pereira, Joana Odila
Guardão, Luísa
Martins, Maria João
Pintado, M. E.
Moura, Daniel
Calhau, Conceição
Pinheiro, Hélder
Palavras-chave: Antihypertensive
Angiotensin-converting enzyme
Bioactive peptides
Mechanisms of action
Data: 2015
Editora: Elsevier
Citação: MARQUES, Cláudia; AMORIM, Maria Manuela; PEREIRA, Joana Odila; GUARDÃO, Luísa; MARTINS, Maria João; PINTADO, M. E.; MOURA, Daniel; CALHAU, Conceição; PINHEIRO, Hélder - In vitro ACE-inhibitory peptide KGYGGVSLPEW facilitates noradrenaline release from sympathetic nerve terminals: Relationship with the lack of antihypertensive effect on spontaneous hypertensive rats. Peptides. ISSN 0196-9781. (2015) vol.71, p.72-76
Resumo: This study aimed to validate the antihypertensive activity of the angiotensin-converting enzyme (ACE)-inhibitor whey protein hydrolysate (WPH) obtained through the action of proteolytic enzymes fromCynara Cardunculus. The antihypertensive activity of WPH fractions containing peptides with molecularweight below 3 kDa (Whey < 3 kDa) and 1 kDa (Whey < 1 kDa) along with the antihypertensive activity ofthree potent ACE-inhibitory peptide sequences (DKVGINYW, DAQSAPLRVY and KGYGGVSLPEW), previ-ously identified in WPH, were also investigated. In parallel, the influence of KGYGGVSLPEW (the mostpotent ACE-inhibitory peptide sequence) on AT1receptors (a common pharmacological target of anti-hypertensive therapies beyond ACE), was evaluated. The effect of WPH and fractions (300 mg/kg) andpeptide sequences (5 mg/kg) on systolic, diastolic and mean blood pressure was evaluated by telemetryon Spontaneously Hypertensive Rats (SHR), after single oral administration. Despite their ACE-inhibitoryeffect in vitro, neither WPH, Whey <3 kDa, Whey <1 kDa or peptide sequences exhibited antihyperten-sive activity. In addition, KGYGGVSLPEW was not only devoid of AT1receptor antagonism but, on thecontrary, had a similar effect to that of Ang II by facilitating the noradrenaline release from sympatheticnerve terminals. In vitro ACE blockade does not always correlate with antihypertensive activity and food-derived peptides cannot be classified as antihypertensive agents based exclusively on in vitro assays. Theabsence of an antihypertensive effect may also be a result of the interaction of these compounds withother components of the systems involved in the blood pressure control.
Peer review: yes
URI: http://hdl.handle.net/10400.14/18850
DOI: http://dx.doi.org/10.1016/j.peptides.2015.06.005
Aparece nas colecções:CBQF - Artigos em revistas (no prelo) / Papers in journals (in press)

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