Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/9997
Título: Wool-associated proteolytic bacteria, isolated from Portuguese Merino breed
Autor: Queiroga, A. Catarina
Pintado, M. E.
Malcata, F. Xavier
Palavras-chave: Sheep
Wool microflora
Caseinolytic activity
Data: 2013
Editora: Elsevier
Citação: QUEIROGA, A. Catarina; PINTADO, M. E.; MALCATA, F. Xavier - Wool-associated proteolytic bacteria, isolated from Portuguese Merino breed. Small Ruminant Research. ISSN 0921-4488. Vol. 109, n.º 1 (2013), p. 38–46
Resumo: The main purpose of this study was to isolate and briefly characterize proteolytic bacteria from a poorly known habitat – raw wool. Fleece samples were accordingly collected from Merino raw wool – a Portuguese ewe breed, at three distinct areas of their body, from animals exhibiting no symptoms or signs of abnormalities; they were then subjected to enumeration and isolation of a total of 158 bacterial strains. Said isolates were screened for protease activity, using the spot technique, on Calcium Caseinate Agar containing 1% (w/v) skim milk. The 36 isolates displaying the highest protease activity underwent a more refined assessment of enzymatic performance – by examining their cell-free supernatant extracts, using casein as substrate. Two Bacillus isolates were eventually chosen owing to their highest proteolytic activities (24.6 and 15.9 U/mL), and identified using molecular biology tools.
Peer review: yes
URI: http://hdl.handle.net/10400.14/9997
Versão do Editor: http://dx.doi.org/10.1016/j.smallrumres.2012.09.005
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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