Orientador(es)
Resumo(s)
The purpose of this study was to compare
the response to infestation by the pine wood nematode
(PWN) Bursaphelenchus xylophilus between
Pinus pinaster and P. pinea at an initial stage of the
disease, 3 h after inoculation. The PWN is the causal
agent of pine wilt disease and is destroying pine
forests all over the world. In Portugal its main
host is the maritime pine, P. pinaster, and its vector
is the longhorn beetle Monochamus galloprovincialis.
Interestingly, this disease does not seem to affect the
species P. pinea and several factors could be behind
this difference in susceptibility. With regards to the
effects of the disease at a transcriptional level, the
suppression subtractive hybridization (SSH) technique
was utilized to identify Expressed Sequence Tags
(EST) in P. pinaster and P. pinea when inoculated
with PWN. EST were isolated, cloned, sequenced
and identified using BlastN and BlastX, and
indicated that at an initial stage of the disease there
is activation of a tree defence response at a
molecular level, mainly related to oxidative stress,
production of lignin and ethylene and posttranscriptional
regulation of nucleic acids. 58% of
the isolated sequences are not yet described, which
shows the lack of genomic information currently
available for pine.
Descrição
Palavras-chave
Early infection Bursaphelenchus EST SSH Stone pine
Contexto Educativo
Citação
SANTOS, Carla S.; VASCONCELOS, M. W. - Identification of genes differentially expressed in Pinus pinaster and Pinus pinea after infection with the pine wood nematode. European Journal of Plant Pathology. ISSN 1573-8469. Vol. 132, n.º 3 (2012), p. 407–418
Editora
Springer