Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/9986
Título: Sequential injection system for the enzymatic determination of ethanol in alcoholic beverages with in-line dilution
Autor: Pais, Teresa F. M.
Vidigal, Susana
Tóth, Ildikó V.
Rangel, António O. S. S.
Palavras-chave: Sequential injection analysis system
Ethanol determination
Wine
Alcohol dehydrogenase
Gas-diffusion unit
Spectrophotometry
Data: 2013
Editora: Elsevier
Citação: PAIS, Teresa F. M... [et al.] - Sequential injection system for the enzymatic determination of ethanol in alcoholic beverages with in-line dilution. Food Control. ISSN 0956-7135. Vol. 30, n.º 2 (2013), p. 616-620
Resumo: A sequential injection system for the enzymatic determination of ethanol in wines with spectrophotometric detection was developed. The determination was based on the enzymatic reaction involving ethanol and NADþ in the presence of alcohol dehydrogenase. A gas-diffusion unit was introduced in the system to perform an in-line dilution. It was possible to operate the system with a reagent consumption of 1.3 mg of NADþ, 0.45 U of ADH, and 32 mL of sample, per assay with a determination rate of 21 h 1. The results obtained from the analysis of wine samples were statistically equivalent to those obtained by the reference method, with good repeatability; the relative standard deviation (n ¼ 10) was <1% with a LOQ of 0.3% (v/v).
Peer review: yes
URI: http://hdl.handle.net/10400.14/9986
Versão do Editor: http://dx.doi.org/10.1016/j.foodcont.2012.08.013
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
AI17.pdf287,92 kBAdobe PDFVer/Abrir    Acesso Restrito. Solicitar cópia ao autor!


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.