Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/9984
Título: Use of solid phase extraction for the sequential injection determination of alkaline phosphatase activity in dynamic water systems
Autor: Santos, Inês C.
Mesquita, Raquel
Bordalo, Adriano A.
Rangel, António O.S.S.
Palavras-chave: Alkaline phosphatase activity
In line solid phase extraction
Sequential injection analysis
NTA Superflow resin
Spectrophotometry
Data: 2012
Editora: Elsevier
Citação: SANTOS, Inês C. ...[et al.] - Use of solid phase extraction for the sequential injection determination of alkaline phosphatase activity in dynamic water systems. Talanta. ISSN 0039-9140. Vol. 98, n.º 30 ( 2012), p. 203–210
Resumo: In this work, a solid phase extraction sequential injection methodology for the determination of alkaline phosphatase activity in dynamic water systems was developed. The determination of the enzymatic activity was based on the spectrophotometric detection of a coloured product, p-nitrophenol, at 405 nm. The p-nitrophenol is the product of the catalytic decomposition of p-nitrophenyl phosphate, a noncoloured substrate. Considering the low levels expected in natural waters and exploiting the fact of alkaline phosphatase being a metalloprotein, the enzyme was pre-concentrated in-line using a NTA Superflow resin charged with Zn2þ ions. The developed sequential injection method enabled a quantification range of 0.044–0.441 unit mL 1 of enzyme activity with a detection limit of 0.0082 unit mL 1 enzyme activity (1.9 mmol L 1 of pNP) and a determination rate of 17 h 1. Recovery tests confirmed the accuracy of the developed sequential injection method and it was effectively applied to different natural waters and to plant root extracts.
Peer review: yes
URI: http://hdl.handle.net/10400.14/9984
Versão do Editor: http://dx.doi.org/10.1016/j.talanta.2012.06.071
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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