Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/9964
Título: Saccharomyces cerevisiae Oxidative Response Evaluation by Cyclic Voltammetry and Gas Chromatography−Mass Spectrometry
Autor: Castro, Cristiana C.
Gunning, Caitriona
Oliveira, Carla M.
Couto, José A.
Teixeira, José A.
Martins, Rui C.
Silva Ferreira, António César
Palavras-chave: Bioanalytical methods
Electrochemical sensors
Mass spectrometry
Data: 2012
Editora: American Chemical Society
Citação: CASTRO, Cristiana C... [et al.] - Saccharomyces cerevisiae Oxidative Response Evaluation by Cyclic Voltammetry and Gas Chromatography−Mass Spectrometry. Journal of Agricultural and Food Chemistry. ISSN 1520-5118. Vol. 60, n.º 29 (2012), p. 7252−7261
Resumo: This study is focused on the evaluation of the impact of Saccharomyces cerevisiae metabolism in the profile of compounds with antioxidant capacity in a synthetic wine during fermentation. A bioanalytical pipeline, which allows for biological systems fingerprinting and sample classification by combining electrochemical features with biochemical background, is proposed. To achieve this objective, alcoholic fermentations of a minimal medium supplemented with phenolic acids were evaluated daily during 11 days, for electrochemical profile, phenolic acids, and the volatile fermentation fraction, using cyclic voltametry, high-performance liquid chromatography−diode array detection, and headspace/solid-phase microextraction/gas chromatography−mass spectrometry (target and nontarget approaches), respectively. It was found that acetic acid, 2- phenylethanol, and isoamyl acetate are compounds with a significative contribution for samples metabolic variability, and the electrochemical features demonstrated redox-potential changes throughout the alcoholic fermentations, showing at the end a similar pattern to normal wines. Moreover, S. cerevisiae had the capacity of producing chlorogenic acid in the supplemented medium fermentation from simple precursors present in the minimal medium
Peer review: yes
URI: http://hdl.handle.net/10400.14/9964
Versão do Editor: dx.doi.org/10.1021/jf300389v
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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