Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/7964
Título: Optimisation, by response surface methodology, of degree of hydrolysis and antioxidant and ACE-inhibitory activities of whey protein hydrolysates obtained with cardoon extract
Autor: Tavares, T. G.
Contreras, M. M.
Amorim, M.
Martín-Álvarez, P. J.
Pintado, M. E.
Recio, I.
Malcata, F. X.
Data: 2011
Editora: Elsevier
Citação: TAVARES, T. G... [et al.] - Optimisation, by response surface methodology, of degree of hydrolysis and antioxidant and ACE-inhibitory activities of whey protein hydrolysates obtained with cardoon extract. International Dairy Journal. ISSN 0958-6946. Vol. 21, n.º 12 (2011), p. 926-933
Resumo: The hydrolysis of bovine whey protein concentrate (WPC), a-lactalbumin (a-La) and caseinomacropeptide (CMP), by aqueous extracts of Cynara cardunculus, was optimized using response surface methodology. Degree of hydrolysis (DH), angiotensin-converting enzyme (ACE)-inhibitory activity and antioxidant activity were used as objective functions, and hydrolysis time and enzyme/substrate ratio as manipulated parameters. The model was statistically appropriate to describe ACE-inhibitory activity of hydrolysates from WPC and a-La, but not from CMP.Maximum DH was 18% and 9%, for WPC and a-La, respectively. 50% ACE-inhibitionwas produced by 105.4 (total fraction) and 25.6 mgmL-1 (<3 kDa fraction) for WPC, and 47.6 (total fraction) and 22.5 mgmL-1 (<3 kDa fraction) for a-La. Major peptides of fractions exhibiting ACEinhibition were sequenced. The antioxidant activities of WPC and a-La were 0.96 +/- 0.08 and 1.12 +/- 0.13 mmol trolox equivalent per mg hydrolysed protein, respectively.
Peer review: yes
URI: http://hdl.handle.net/10400.14/7964
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0958694611001774
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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