Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/7963
Título: Novel whey-derived peptides with inhibitory effect against angiotensin-converting enzyme: in vitro effect and stability to gastrointestinal enzymes
Autor: Tavares, Tânia
Del Mar Contreras, Maria
Amorim, Manuela
Pintado, Manuela
Recio, Esidra
Malcata, F. Xavier
Palavras-chave: ACE-inhibitory peptide
Gastrointestinal digestion
Hydrolyzed whey
Antihypertensive activity
Data: 2011
Editora: Elsevier
Citação: TAVARES, Tânia... [et al.] - Novel whey-derived peptides with inhibitory effect against angiotensin-converting enzyme: in vitro effect and stability to gastrointestinal enzymes. Peptides. ISSN 0196-9781. Vol. 32, n.º 5 (2011), p. 1013-1019
Resumo: Whey protein concentrate (WPC) was subjected to enzymatic hydrolysis by proteases from the flowers of Cynara cardunculus, and the resulting angiotensin-converting enzyme (ACE)-inhibitory effect was monitored. The whole WPC hydrolysate exhibited an IC50 value of 52.9±2.9ºg/mL, whereas the associated peptide fraction with molecular weight below 3 kDa scored 23.6±1.1ºg/mL. The latter fraction was submitted to RP-HPLC, and 6 fractions were resolved that exhibited ACE-inhibitory effects. Among the various peptides found, a total of 14 were identified via sequencing with an ion-trap mass spectrometer. Eleven of these peptides were synthesized de novo – to validate their ACE-inhibitory effect, and also to ascertain their stability when exposed to simulated gastrointestinal digestion. Among them, three novel, highly potent peptides were found, corresponding to B-lactalbumin f(16–26) – with the sequence KGYGGVSLPEW, B-lactalbumin f(97–104) with DKVGINYW, and B-lactoglobulin f(33–42) with DAQSAPLRVY; their IC50 values were as low as 0.80±0.1, 25.2±1.0 and 13.0±1.0g/mL, respectively. None of them remained stable in the presence of gastrointestinal enzymes: they were partially, or even totally hydrolyzed to smaller peptides – yet the observed ACE-inhibitory effects were not severely affected for two of those peptides.
Peer review: yes
URI: http://hdl.handle.net/10400.14/7963
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S019697811100074X
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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