Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/7952
Título: Cytotoxicity and genotoxicity of chitooligosaccharides upon lymphocytes
Autor: Fernandes, João C.
Borges, Margarida
Nascimento, Henrique
Bronze-da-Rocha, Elsa
Ramos, Oscar S.
Pintado, Manuela E.
Malcata, F. Xavier
Santos-Silva, Alice
Palavras-chave: Micronucleus
Comet assay
Flow cytometry
Chitooligosaccharides
Lymphocytes
Data: 2011
Editora: Elsevier
Citação: FERNANDES, João C... [et al.] - Cytotoxicity and genotoxicity of chitooligosaccharides upon lymphocytes. International Journal of Biological Macromolecules. ISSN 0141-8130. Vol. 49, n.º3 (2011), p. 433-438
Resumo: Two COS mixtures and a low molecular weight chitosan (LMWC) were tested for potential cytotoxicity and genotoxicity upon human lymphocytes. Genotoxicity was evaluated in vitro by cytokinesis-blocked micronucleus and alkaline comet assays, while cytotoxicity was assessed by flow cytometry analysis. Our results suggest that COS do not exhibit any genotoxicity upon human lymphocytes, independently of MW or concentration. However, above 0.07 mg/mL COS induced strong cytotoxic effects. According to the concentration used, such cytotoxicity will induce cell death, essentially by necrosis (>0.10 mg/mL) and/or apoptosis (<0.10 mg/mL). The level of necrosis/apoptosis induced by high COS concentrations, suggests a promising use as apoptosis inducers in specific cancer situations.
Peer review: yes
URI: http://hdl.handle.net/10400.14/7952
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0141813011002273
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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