| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 300.62 KB | Adobe PDF |
Advisor(s)
Abstract(s)
Aims: To evaluate the effect of wine phenolic compounds on the production of
volatile phenols (4-vinylphenol [4VP] and 4-ethylphenol [4EP]) from the
metabolism of p-coumaric acid by lactic acid bacteria (LAB).
Methods and Results: Lactobacillus plantarum, Lactobacillus collinoides and
Pediococcus pentosaceus were grown in MRS medium supplemented with
p-coumaric acid, in the presence of different phenolic compounds: nonflavonoids
(hydroxycinnamic and benzoic acids) and flavonoids (flavonols and
flavanols). The inducibility of the enzymes involved in the p-coumaric acid
metabolism was studied in resting cells. The hydroxycinnamic acids tested
stimulated the capacity of LAB to synthesize volatile phenols. Growth in the
presence of hydroxycinnamic acids, especially caffeic acid, induced the production
of 4VP by resting cells. The hydroxybenzoic acids did not significantly
affect the behaviour of the studied strains. Some of the flavonoids showed an
effect on the production of volatile phenols, although strongly dependent on
the bacterial species. Relatively high concentrations (1 g l)1) of tannins inhibited
the synthesis of 4VP by Lact. plantarum.
Conclusions: Hydroxycinnamic acids were the main compounds stimulating
the production of volatile phenols by LAB. The results suggest that caffeic and
ferulic acids induce the synthesis of the cinnamate decarboxylase involved in
the metabolism of p-coumaric acid. On the other hand, tannins exert an inhibitory
effect.
Significance and Impact of the Study: This study highlights the capacity of
LAB to produce volatile phenols and that this activity is markedly influenced
by the phenolic composition of the medium.
Description
Keywords
Lactic acid bacteria p-coumaric acid Phenolic compounds Volatile phenols Wine
Pedagogical Context
Citation
SILVA, I....[et al] - Wine phenolic compounds influence the production of volatile phenols by wine-related lactic acid bacteria. Journal of Applied Microbiology. ISSN 1364-5072. Vol. 111 (2011), p. 360–370
Publisher
Wiley Blackwell