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Resposta fisiológica de pinus spp. nas primeiras horas após infecção com bursaphelenchus xylophilus (nematoda: aphelenchoididae)
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Orientador(es)
Resumo(s)
O objectivo deste estudo consistiu na comparação da resposta à infecção com o nemátode da madeira do pinheiro (NMP) Bursaphelenchus xylophilus entre Pinus pinaster e Pinus pinea no estádio inicial da doença, três horas após inoculação. O NMP é o agente causal da doença da madeira do pinheiro, tem em Portugal como hospedeiro principal o pinheiro bravo, P. pinaster, e tem como vector Monochamus galloprovincialis. Actualmente, não há estudos que descrevam os efeitos da infecção do nemátode nas plantas a um estádio inicial da doença e, no que diz respeito aos efeitos fisiológicos e metabólicos nas plantas, verificou-se uma diminuição de 10,9% no teor de água em P. pinaster (por comparação com árvores inoculadas com água) e 6,7% em P. pinea; relativamente à concentração de clorofilas, uma diminuição para cerca de metade do valor controlo foi registado para ambas as espécies, mas mais pronunciadamente em P. pinaster. Um estudo exploratório com cromatografia gasosa também foi efectuado, que demonstrou que os compostos voláteis produzidos permitem uma clara diferenciação entre espécies, mas não entre inoculação controlo vs. nemátode.
The purpose of this study was to compare the response to infection with the pine wood nematode (PWN) Bursaphelenchus xylophilus between Pinus pinaster and Pinus pinea at an initial stage of the disease, three hours after inoculation. The PWN is the causal agent of pine wilt disease, in Portugal its main host is the maritime pine, P. pinaster, and its vector is Monochamus galloprovincialis. Currently there are no studies that describe the effects of nematode infection in plants at an early stage of the disease and with regards to the physiological and metabolic indicators of the disease response in the plants, there was a decrease of 10.9% of water content in P. pinaster (compared with trees inoculated with water) and 6.7% in P. pinea; in the study of total chlorophyll concentration, a decrease to about half of the control value was recorded for both species, but more pronounced in P. pinaster. An exploratory study was also made with gas chromatography, which showed that the volatile compounds produced were distinct between species, but not between control vs. nematode-inoculated plants.
The purpose of this study was to compare the response to infection with the pine wood nematode (PWN) Bursaphelenchus xylophilus between Pinus pinaster and Pinus pinea at an initial stage of the disease, three hours after inoculation. The PWN is the causal agent of pine wilt disease, in Portugal its main host is the maritime pine, P. pinaster, and its vector is Monochamus galloprovincialis. Currently there are no studies that describe the effects of nematode infection in plants at an early stage of the disease and with regards to the physiological and metabolic indicators of the disease response in the plants, there was a decrease of 10.9% of water content in P. pinaster (compared with trees inoculated with water) and 6.7% in P. pinea; in the study of total chlorophyll concentration, a decrease to about half of the control value was recorded for both species, but more pronounced in P. pinaster. An exploratory study was also made with gas chromatography, which showed that the volatile compounds produced were distinct between species, but not between control vs. nematode-inoculated plants.
Descrição
Palavras-chave
Concentração de clorofilas Conteúdo de água Cromatografia gasosa Chlorophyll concentration Water content Gas chromatography
Contexto Educativo
Citação
SANTOS, Carla; VASCONCELOS, Marta - Resposta fisiológica de pinus spp. nas primeiras horas após infecção com bursaphelenchus xylophilus (nematoda: aphelenchoididae). Silva Lusitana. ISSN 0870-6352. Vol. 19, n.º1 (2011), p. 99-110
Editora
Instituto Nacional de Recursos Biológicos, Instituto Nacional de Investigação Agrária