Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/7642
Título: Autolysis of cell walls from polygalacturonase-antisense tomato fruit in simulated apoplastic solutions
Autor: Almeida, Domingos P.F.
Huber, Donald J.
Palavras-chave: Cell wall
Fruit ripening
Pectin
Solanum lycopersicum
Uronic acid
Data: 2011
Editora: Elsevier
Citação: ALMEIDA, Domingos P.F; HUBER, Donald J. - Autolysis of cell walls from polygalacturonase-antisense tomato fruit in simulated apoplastic solutions. Plant Physiology and Biochemistry. ISSN 0981-9428. Vol. 49, n.º 6 (2011), p. 617-622
Resumo: Autolysis of cell walls from polygalacturonase (PG)-antisense tomato fruit was studied in a conventional buffer designed to maximize the catalytic activity of PG (30 mM sodium acetate, 150 mM NaCl, pH 4.5), and in solutions mimicking the pH and mineral composition of the fruit apoplast at the mature-green and ripe stages. Autolytic release of uronic acids was very limited under simulated apoplastic conditions compared with the conventional buffer, but minimal differences in the release of reducing groups were observed among the incubation conditions. Autolytic release of uronic acids from active walls was lower than solubilization from enzymically inactive walls. Uronic acids that remained ionically bound to the cell walls during autolysis were subsequently extracted and analyzed by size exclusion chromatography. The elution profiles of ionically bound uronic acids from cell walls incubated under optimal conditions were similar for all ripening stages. In solutions mimicking the pH and mineral composition of the apoplast of mature-green and ripe fruit, uronic acids extracted from pink and ripe fruit cell walls showed a decrease in average molecular mass compared with polymers from mature-green cell walls. The results suggest that the composition of the incubation solution exert strong influence on PG-independent cell wall autolysis and that enzymically active walls restrain PG-independent pectin solubilization.
Peer review: yes
URI: http://hdl.handle.net/10400.14/7642
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0981942811000775
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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