Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/7639
Título: Differential responses of the antioxidant defence system and ultrastructure in a salt-adapted potato cell line
Autor: Queirós, Filipa
Rodrigues, José A.
Almeida, José M.
Almeida, Domingos P. F.
Fidalgo, Fernanda
Palavras-chave: Antioxidant defence system
Callus tissue
Electron microscopy
Oxidative stress
Salinity
Solanum tuberosum
Data: 2011
Editora: Elsevier
Citação: QUEIRÓS, Filipa... [et al.] - Differential responses of the antioxidant defence system and ultrastructure in a salt-adapted potato cell line. Plant Physiology and Biochemistry. ISSN 0981-9428. Vol. 49, n.º 12 (2011), p. 1410-1419
Resumo: Changes in lipid peroxidation and ion content and the possible involvement of the antioxidant system in salt tolerance at the cellular level was studied in a potato (Solanum tuberosum L.) callus line grown on 150 mM NaCl (salt-adapted) and in a non-adapted line exposed to 150 mM NaCl (salt-stressed). Salinity reduced the growth rate and increased lipid peroxidation in salt-stressed line, which remained unaltered in the adapted line. Na+ and Cl− content increased due to salinity in both lines, but the adapted line displayed greater K+/Na+ ratio than the stressed one. Total superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), and glutathione reductase (GR, EC 1.6.4.2) activities decreased in both salt-exposed lines; catalase (CAT, EC 1.11.1.6) activity did not change in the adapted line, but decreased in the stressed cell line. Salinity caused the suppression of one GR isoform, while the isozyme patterns of SOD, APX, and CAT were not affected. Ascorbate and reduced glutathione increased in both salt-exposed calli lines. α-Tocopherol increased as a result of salt exposure, with higher levels found in adapted calli. Electron microscopy showed that neither the structural integrity of the cells nor membrane structure were affected by salinity, but plastids from adapted cells had higher starch content. The results suggest that the enzymic and non-enzymic components of the antioxidant system are differentially modulated by salt. Different concentrations of antioxidant metabolites are more relevant to the adaptive response to salinity in potato calli than the differences in activity of the antioxidant enzymes.
Peer review: yes
URI: http://hdl.handle.net/10400.14/7639
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0981942811002889
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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