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|Título:||Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol|
|Autor:||Duque, Anouk F.|
Hasan, Syed A.
Bessa, Vânia S.
Carvalho, Maria F.
Janssen, Dick B.
Castro, Paula M. L.
|Citação:||DUQUE, Anouk F... [et al.] (2012) - Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol. Applied Microbiology and Biotechnology. ISSN 0175-7598. Vol.95, nº2 (2012) p.511-520|
|Resumo:||A pure bacterial culture able to utilize 2- fluorophenol (2-FP) as sole carbon and energy source was isolated by selective enrichment from sediments collected from a contaminated site in Northern Portugal. 16S rRNA gene analysis showed that the organism (strain FP1) belongs to the genus Rhodococcus. When grown aerobically on 2-FP, growth kinetics of strain FP1 followed the Luong model. An inhibitory effect of increasing 2-FP concentrations was observed with no growth occurring at 2- FP levels higher than ca. 4 mM. Rhodococcus strain FP1 was able to degrade a range of other organofluorine compounds, including 2-fluorobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-fluorophenol, 4-fluorophenol, 3-fluorocatechol, and 4-fluorocatechol, as well as chlorinated compounds such as 2-chlorophenol and 4-chlorophenol. Experiments with cell-free extracts and partially purified enzymes indicated that the first step of 2-fluorophenol metabolism was conversion to 3-fluorocatechol, suggesting an unusual pathway for fluoroaromatic metabolism. To our knowledge, this is the first time that utilization of 2-FP as a growth substrate by a pure bacterial culture is reported.|
|Versão do Editor:||The original publication is available at http://www.springerlink.com/content/wuq51847215278j6/abstract/|
|Aparece nas colecções:||CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review|
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|Duque et al 2011c.1.pdf||571,62 kB||Adobe PDF||Ver/Abrir|
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