Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/7457
Título: Culture-dependent and culture-independent diversity surveys target different bacteria: a case study in a freshwater sample
Autor: Vaz-Moreira, Ivone
Egas, Conceição
Nunes, Olga C.
Manaia, Célia M.
Palavras-chave: Culture-dependent
Cultureindependent
DGGE 454 Pyrosequencing
Freshwater
Bacterial diversity
Data: 2011
Editora: Springer
Citação: VAZ-MOREIRA, Ivone...[et al] - Culture-dependent and culture-independent diversity surveys target different bacteria: a case study in a freshwater sample. Antonie van Leeuwenhoek. ISSN 1572-9699. Vol. 100, n.º 2 (2011), p. 245-257
Resumo: Compared with culture-independent approaches, traditionally used culture-dependent methods have a limited capacity to characterizewatermicrobiota. Nevertheless, for almost a century the latter have been optimized to detect and quantify relevant bacteria. A pertinent question is if culture-independent diversity surveys give merely an extended perspective of the bacterial diversity or if, even with a higher coverage, focus on a different set of organisms. We compared the diversity and phylogeny of bacteria in a freshwater sample recovered by currently used culture-dependent and culture-independent methods (DGGE and 454 pyrosequencing). The culture-dependent diversity survey presented lower coverage than the other methods. However, it allowed bacterial identifications to the species level, in contrast with the other procedures that rarely produced identifications below the order. Although the predominant bacterial phyla detected by both approaches were the same (Proteobacteria, Actinobacteria, Bacteroidetes), sequence similarity analysis showed that, in general, different operational taxonomical units were targeted by each method. The observation that culture-dependent and independent approaches target different organisms has implications for the use of the latter for studies in which taxonomic identification has a predictive value. In comparison to DGGE, 454 pyrosequencing method had a higher capacity to explore the bacterial richness and to detect cultured organisms, being also less laborious.
Peer review: yes
URI: http://hdl.handle.net/10400.14/7457
Versão do Editor: The original publication is available at http://www.springerlink.com/content/0003-6072/
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review



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