Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/7418
Título: A molecular and multivariate approach to the microbial community of a commercial shallow raceway marine recirculation system operating with a moving bed biofilter
Autor: Matos, Ana
Borges, Maria-Teresa
Peixe, Carla
Henriques, Isabel
Pereira, Carlos M.
Castro, Paula M. L.
Palavras-chave: Microbial community
MBBR
Shallow raceway system
DGGE
Multivariate analysis
Data: 2011
Editora: Wiley Blackwell
Citação: MATOS, Ana... [et al.] - A molecular and multivariate approach to the microbial community of a commercial shallow raceway marine recirculation system operating with a moving bed biofilter. Aquaculture Research. ISSN 1355-557X. Vol. 42, n.º 9 (2011), p. 1308-1322
Resumo: Information on commercial aquaculture systems bacterial communities is scarce. The present study was performed in a turbot shallow raceway recirculating aquaculture system throughout 221days after initial fish stocking. The dynamics of bacteria attached to a Moving Bed Biofilm Reactor and the distribution of the system culturable planktonic heterotrophic bacteria were assessed. Diferent levels of free heterotrophic bacteria were found over time: ≤ 2.6 x 104 CFUmL 1 in incoming and ozonated water and 1.5 106 CFUmL 1 in raceway and bio¢lter water. 16S rRNA gene sequencing of nine strains persistently isolated fromthe systema⁄liated with Bacteroidetes, a-Proteobacteria or g-Proteobacteria. Polymerase chain reaction-denaturating gradient gel electrophoresis (PCR-DGGE) data showed temporal and spatial segregation of attached biofilter community. The first 32 days were the most important for bacterial establishment with higher culturable bacteria biomass loadings and higher community diversity. Multivariate canonical correspondence analysis of PCR-DGGE showed that the most common aquaculture water quality descriptors explained 70% of the DGGE pattern variability.
Peer review: yes
URI: http://hdl.handle.net/10400.14/7418
Versão do Editor: The definitive version is available at http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2010.02719.x/abstract
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review



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