Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6908
Título: Efficiency of four secondary enrichment protocols in differentiation and isolation of Listeria spp. and Listeria monocytogenes from smoked fish processing chains
Autor: Duarte, Gabriela
Vaz-Velho, Manuela
Capella, Christopher
Gibbs, Paul
Palavras-chave: Cold-smoked fish
Listeria
Listeria monocytogenes
Secondary selective enrichments
Conventional cultural and bactometer capacitance-based detection methods
Data: 1999
Editora: Elsevier
Citação: DUARTE, Gabriela... [et al.] - Efficiency of four secondary enrichment protocols in differentiation and isolation of Listeria spp. and Listeria monocytogenes from smoked fish processing chains. International Journal of Food Microbiology. Vol. 52, n.º 3 (1999), p. 163–168
Resumo: Four secondary enrichment protocols (conventional methods: UVM II, Fraser 24 h and Fraser 48 h: Impedimetric method: Listeria electrical detection medium) were studied for their ability to isolate Listeria spp. and Listeria monocytogenes from fish and environmental samples collected along the processing chain of cold-smoked fish. From all methods, Listeria spp. and L. monocytogenes were respectively present in 56 and 34 of 315 samples analysed. Fraser broth incubated for 48 h gave the fewest false negative Listeria spp. results [4/56; (7.1%)], but concurrently only 15/34 (44.1%) samples were correctly identified as containing L. monocytogenes. Listeria electrical detection (LED) medium detected only 36/56 (64.3%) Listeria spp. positive samples. Despite this lower isolation rate, LED identified 20/34 (58.8%) L. monocytogenes positive samples correctly and gave fewer false positive results. The overall conclusion was that more than one isolation method is needed to accurately estimate L. monocytogenes contamination rates.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6908
Versão do Editor: doi:10.1016/S0168-1605(99)00121-X
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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