Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6900
Título: Caseinolytic activity of fruit extract from Opuntia ficus-indica on bovine, caprine, and ovine Sodium Caseinates
Autor: Pintado, Ana I.
Macedo, Angela C.
Teixeira, Grimaneza
Pais, Salomé
Clemente, Alda
Malcata, F. Xavier
Data: 2001
Editora: American Chemical Society e American Institute of Chemical Engineers
Citação: PINTADO, Ana I...[et al.] - Caseinolytic Activity of Fruit Extract from Opuntia ficus-indica on Bovine, Caprine, and Ovine Sodium Caseinates. Biotechnology Progress. ISSN 8756-7938. Vol. 17, n.º 4 (2001), p. 643-646
Resumo: The rates and extents of hydrolysis of RS- and â-caseins from bovine, caprine, and ovine sodium caseinates produced by an enzymatic extract of the fruit of Opuntia ficusindica, (L.) Miller were evaluated and compared with those produced by a commercial animal rennet. A mechanistic model based on a pseudo-first-order enzymatic reaction, in the presence of first-order deactivation of the enzyme, was postulated and successfully fitted to the experimental data. The animal rennet exhibited higher enzymatic efficiency than the fruit extract, irrespective of the source (i.e., bovine, caprine, or ovine) and the type (i.e., RS- or â-casein) of substrate. The enzymatic efficiency (kcat/Km) for RS-casein ranged from 72 to 220 and from 43 to 65 L g-1 h-1, and for â-casein from 242 to 742 and from 55 to 164 L g-1 h-1, for the animal rennet and the enzymatic extract of O. ficus-indica, respectively. Finally, it was observed that â-casein from caprine and ovine caseinates was degraded by O. ficus-indica faster than its RS counterpart, but the reverse was observed for bovine caseinate.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6900
Versão do Editor: The definitive version is available at www3.interscience.wiley.com
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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