Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6829
Título: Production of histamine and tyramine by bacteria isolated from Portuguese vacuum-packed cold-smoked fish
Autor: Silva, Manuela V. da
Pinho, Olívia
Ferreira, Isabel
Plestilov, Linda
Palavras-chave: Histamine
Tyramine
Biogenic amines
Decarboxylating bacteria
Cold-smoked fish
Data: 2002
Editora: Elsevier
Citação: SILVA, Manuela V. da - Production of histamine and tyramine by bacteria isolated from Portuguese vacuum-packed cold-smoked fish. Food Control. ISSN 0956-7135. Vol. 13 (2002), p. 457–461
Resumo: An agar medium containing histidine or tyrosine incubated anaerobically was used for detecting the decarboxylating activity of bacteria isolated from Portuguese vacuum-packed cold-smoked fish during chilled storage. The capacity of each bacterial isolate to produce histamine and tyramine was studied at 25 and 5 C incubated for 48 h and 10 days, respectively. More strains produced histamine and tyramine at 25 C compared with 5 C although lactic acid bacteria (LAB) strains exhibited similar results at the two different temperatures. Tyramine was produced by majority of the isolates tested although very low concentrations were produced at 5 C as confirmed by high-pressure liquid chromatography (HPLC). Tyrosine-agar was shown to be a good indicator medium for detection of bacteria that produced high levels of tyramine, since typical colonies surrounded by a translucent halo were easily recognised. LAB identified as Lc. Lactis lactis 1 and Carnobacterium divergens were detected as tyramine-producing bacteria. Acinetobacter spp. and Pseudomonas spp., isolated from all Portuguese smoked fish products, were negative on histidine-agar, but HPLC identified considerable quantities of histamine produced in a broth medium.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6829
Versão do Editor: doi:10.1016/S0956-7135(01)00081-0
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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