Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6820
Título: Kinetic determination of Image (−)malic acid in wines using sequential injection analysis
Autor: Segundo, Marcela A.
Rangel, António O. S. S.
Palavras-chave: Sequential injection analysis
Malic acid
Kinetic enzymatic determination
Data: 2003
Editora: Elsevier
Citação: SEGUNDO, Marcela A. ; RANGEL, António O.S.S. - Kinetic determination of Image (−)malic acid in wines using sequential injection analysis. Analytica Chimica Acta. ISSN 0003-2670. Vol. 499, n.º1-2 (2003) p. 99-106
Resumo: In the present work a sequential injection system for the enzymatic determination of Image (−)malic acid in wines is described. The determination was based on the detection of NADH, formed by oxidation of Image -malate to oxaloacetate, catalysed by Image -malate dehydrogenase. A kinetic method was implemented by stopping the flow when a solution plug containing sample, NAD+ and enzyme reached the detector. The rate of reaction was measured by monitoring absorbance changes at 340 nm during a fixed period of time. A linear calibration curve (rate of reaction versus concentration) was obtained for concentrations of Image (−)malic acid between 0.01 and 0.15 g l−1. The determination rate was about 22 h−1 and samples were diluted 25 or 50 times before introduction to the system. Results obtained from 16 wine samples were comparable to those obtained by the reference method.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6820
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0003267003008857
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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