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|Título:||Kinetic determination of Image (−)malic acid in wines using sequential injection analysis|
|Autor:||Segundo, Marcela A.|
Rangel, António O. S. S.
|Palavras-chave:||Sequential injection analysis|
Kinetic enzymatic determination
|Citação:||SEGUNDO, Marcela A. ; RANGEL, António O.S.S. - Kinetic determination of Image (−)malic acid in wines using sequential injection analysis. Analytica Chimica Acta. ISSN 0003-2670. Vol. 499, n.º1-2 (2003) p. 99-106|
|Resumo:||In the present work a sequential injection system for the enzymatic determination of Image (−)malic acid in wines is described. The determination was based on the detection of NADH, formed by oxidation of Image -malate to oxaloacetate, catalysed by Image -malate dehydrogenase. A kinetic method was implemented by stopping the flow when a solution plug containing sample, NAD+ and enzyme reached the detector. The rate of reaction was measured by monitoring absorbance changes at 340 nm during a fixed period of time. A linear calibration curve (rate of reaction versus concentration) was obtained for concentrations of Image (−)malic acid between 0.01 and 0.15 g l−1. The determination rate was about 22 h−1 and samples were diluted 25 or 50 times before introduction to the system. Results obtained from 16 wine samples were comparable to those obtained by the reference method.|
|Versão do Editor:||http://www.sciencedirect.com/science/article/pii/S0003267003008857|
|Aparece nas colecções:||ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review|
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|Kinetic determination of L(-)malic acid in wines using sequential injection analysis.pdf||139,81 kB||Adobe PDF||Ver/Abrir|
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