Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6819
Título: Comparative catalytic activity of two plant proteinases upon caprine caseins in solution
Autor: Silva, Sofia V.
Malcata, F. Xavier
Palavras-chave: Goat
Rennet substitute
Proteolysis
Electrophoresis
RP-HPLC
Data: 2000
Editora: Elsevier
Citação: SILVA Sofia V. ; MALCATA, F. Xavier - Comparative catalytic activity of two plant proteinases upon caprine caseins in solution. Food Chemistry. ISSN 0308-8146. Vol. 71, n.º2 (2000), p. 207-214
Resumo: The proteolytic activities of cardosins A and B, two (plant) proteinases from Cynara cardunculus, toward caprine caseins, independently, or in the presence of each other as Na-caseinate, were studied in a comparative fashion using polyacrylamide gel electrophoresis and reversed phase high performance liquid chromatography. The electrophoretic degradation patterns of both αs- and β-casein, brought about by the cardosins, were similar to one another. In what concerns the specificity of these two enzymes upon caseinate, the major cleavage sites were Leu127-Thr128 and Leu190-Tyr191, both in β-casein. When caseins were tested independently, both cardosins cleaved Phe153-Tyr154 in αs1-casein, as well as Leu127-Thr128 and Leu190-Tyr191 in β-casein.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6819
Versão do Editor: doi:10.1016/S0308-8146(00)00157-6
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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