Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6809
Título: Evaluation of mini-VIDAS rapid test for detection of Listeria monocytogenes from production lines of fresh to cold-smoked fish
Autor: Vaz-Velho, Manuela
Duarte, Gabriela
Gibbs, Paul
Palavras-chave: Cold-smoked fish
Listeria monocytogenes
Rapid immunological-based methods
Data: 2000
Editora: Elsevier
Citação: VAZ-VELHO, Manuela; DUARTE, Gabriela; GIBBS, Paul - of Listeria monocytogenes from production lines of fresh to cold-smoked fish. Journal of Microbiological Methods. ISSN 0167-7012. Vol. 40 (2000), p. 147–151
Resumo: This study was conducted to evaluate the efficacy of the mini-VIDAS Listeria monocytogenes (LMO) system (BioMe´rieux Vitek, Inc., Missouri, USA) for detection of L. monocytogenes in environmental and fish samples from three Portuguese cold-smoking plants and from their fresh fish suppliers. Mini-VIDAS-LMO is a fully automated system that uses fluorescent ELFA (Enzyme Linked Fluorescent Assay) technology for detection of Listeria monocytogenes antigens in food. It can be a rapid screening method alternative to time consuming classical isolation and identification. Two hundred and ninety five samples were tested in mini-VIDAS-LMO and in parallel by the ISO 11290-1 traditional protocol. The mini-VIDAS-LMO detected 8 of the 11 confirmed positive samples and presented 11 false positive results. The specificity of the mini-VIDASLMO found in this experiment was 0.96 and the sensitivity 0.73
Peer review: yes
URI: http://hdl.handle.net/10400.14/6809
Versão do Editor: doi:10.1016/S0167-7012(00)00118-4
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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