Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6808
Título: Hydrolysis of caseins by extracts of Cynara Cardunculus precipitated by ammonium sulfate
Autor: Silva, S.V.
Barros, R.M.
Malcata, F.X.
Palavras-chave: Milk proteins
Thistle
FPLC
Urea-PAGE
Data: 2002
Editora: Wiley-Blackwell
Citação: SILVA, S.V.; BARROS, R.M.; MALCATA, F.X. - Hydrolysis of caseins by extracts of Cynara Cardunculus precipitated by ammonium sulfate. Journal of Food Science. ISSN 0022-1147. Vol. 67, n.º 5 (2002), p. 1746-1751
Resumo: Polyacrylamide gel electrophoresis (in the presence of urea) and gel permeation chromatography were employed to assess the profile of hydrolysis of caseins and the activity of enzymes contributed by the flowers of the plant Cynara cardunculus on bovine caseins, after previous precipitation with ammonium sulfate or in a plain crude aqueous extract. Results indicated that the qualitative degradation profile of bovine caseins by plant enzymes (cardosins) remains essentially unchanged upon extraction, and quantitative analysis showed that the precipitated fractions had a higher coagulant-to-proteolytic activity ratio; hence, the results showed that inexpensive precipitation with ammonium sulfate can successfully be used as a purification method in the production of that plant coagulant in standardized form.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6808
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review



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