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|Título:||Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods|
Pacheco, Catarina C.
Marco, Paolo De
Castro, Paula M.L.
|Editora:||Kluwer Academic Publishers|
|Citação:||BASTOS, Filipe...[et al] - Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods. Biodegradation. ISSN 0923-9820. Vol. 13:(2002), p. 211–220|
|Resumo:||Microbial cultures able to degrade xenobiotic compounds are the key element for biological treatment of waste effluents and are obtained from enrichment processes. In this study, two common enrichment methods, suspension batch and immobilized continuous, were compared. The main selection factor was the presence of 1,3-dichloro- 2-propanol (1,3-DCP) as the single carbon source. Both methods have successfully enriched microbial consortia able to degrade 1,3-DCP. When tested in batch culture, the degradation rates of 1,3-DCP by the two consortia were different, with the consortia obtained by batch enrichment presenting slightly higher rates. A preliminary morphological and biochemical analysis of the predominant colonial types present in each degrading consortia revealed the presence of different constituting strains. Three bacterial isolates capable of degrading 1,3-DCP as single strains were obtained from the batch enrichments. These strains were classified by 16S rRNA analysis as belonging to the Rhizobiaceae group. Degradation rates of 1,3-DCP were lower when single species were used, reaching 45 mg l−1 d−1, as compared to 74 mg l−1 d−1 of the consortia enriched on the batch method. Mutualistic interactions may explain the better performance of the enriched consortia.|
|Versão do Editor:||The original publication is available at www.springerlink.com|
|Aparece nas colecções:||ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review|
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