Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6801
Título: Evaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: effects on astaxanthin recovery and implications for bio-availability
Autor: Mendes-Pinto, M.M.
Raposo, M.F.J.
Bowen, J.
Young, A.J.
Morais, R.
Palavras-chave: Astaxanthin
Bio-availability
Haematococcus
Microalgae
Processing
Data: 2001
Editora: Springel Verlag
Citação: MENDES-PINTO, M.M...[et al.] - Evaluation of different cell disruption processes on encysted cells of Haematococcus pluvialis: effects on astaxanthin recovery and implications for bio-availability. Journal of Applied Phycology. ISSN 1573-5176. Vol. 13, n.º 1 (2001), p. 19–24
Resumo: Although Haematococcus pluvialis is one of the most importantnatural sources of the carotenoid astaxanthin as a pigmentor for theaquaculture industry, the thick sporopollenin cell wall in the cysts hindersastaxanthin extraction and its subsequent bio-availability to fish. A rangeof physical and chemical processes were tested to promote the disruptionof the encysted cells. The efficacy of these processes was evaluated interms of astaxanthin recovery, which was assessed by determining theextent of leaching of astaxanthin into an organic solvent. The processestested were: autoclave 30 min, 121 °C, 1 atm; HCl 0.1 M, 15min and 30 min; NaOH 0.1 M, 15 min and 30 min; enzymatictreatment with a mixture of 0.1% protease K and 0.5% driselase in aphosphate buffer, pH 5.8, 30 °C, for one hour; spray drying, inlet180 °C, outlet 115 °C; and mechanical disruption, with acell homogeniser developed for this purpose. The mechanical(homogenisation) and autoclave treatments were the most effective in termsof extraction and availability.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6801
Versão do Editor: The original publication is available at www.springerlink.com
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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