Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6786
Título: Sequential injection system for the enzymatic determination of ethanol in wine
Autor: Páscoa, Ricardo N. M. J.
Vidigal, Susana S. M. P.
Tóth, Ildikó Varga
Rangel, $bAntónio Osmaro Santos Silva
Palavras-chave: Sequential injection analysis
Alcohol dehydrogenase
Ethanol
Wine
Spectrophotometry
Data: 2006
Editora: American Chemical Society
Citação: "Journal of Agricultural and Food Chemistry" . ISSN 0021-8561. 54:1 (2006) 19-23
Resumo: A sequential injection system was developed for the enzymatic determination of ethanol in wine. The spectrophotometric determination is based on the enzymatic reaction catalyzed by alcohol dehydrogenase in the presence of NAD+. The system was applied to the determination of ethanol in a range of 0.008-0.024% (v/v) with good repeatability; RSDn)10 < 2.3%. The results obtained with the developed system showed good agreement with those obtained by using the reference method. The determination rate was 25 h-1; 1 ímol of NAD+, 1.1 units of enzyme, and 50 íL of sample were consumed per determination; and the waste produced was 2.2 mL per assay.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6786
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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