Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6699
Título: Biodiversity and characterization of Staphylococcus species isolated from a small manufacturing dairy plant in Portugal
Autor: Soares, José C.
Marques, M. Rosário
Tavaria, Freni K.
Pereira, Joana O.
Malcata, F. Xavier
Pintado, Manuela M.
Palavras-chave: Staphylococcus
Multiplex-PCR
ARDRA
Enterotoxin
Antibiotic profile
Virulence factors
Data: 2011
Editora: Elsevier
Citação: SOARES, José C....[et al] - Biodiversity and characterization of Staphylococcus species isolated from a small manufacturing dairy plant in Portugal. International Journal of Food Microbiology. ISSN 0168-1605. Vol. 146 n.º 2 (2011), p. 123–129
Resumo: The level and the diversity of the staphylococcal community occurring in the environment and dairy products of a small manufacturing dairy plant were investigated. Species identification was performed using different molecular methods, viz. Multiplex-PCR, amplified ribosomal DNA restriction analysis (ARDRA), and sodA gene sequencing. The main species encountered corresponded to Staphylococcus equorum (41 isolates, 39.0%), S. saprophyticus (28 isolates, 26.7%) and S. epidermidis (15 isolates, 14.3%). Additionally, low incidence of enterotoxin genes was obtained, with only 9 strains (8.6%) being positive for one or more toxin genes. With regard to antimicrobial resistance, 57.1% of the isolates showed at least resistance against one antibiotic, and 28.6% were multi-resistant, which might accomplish resistance for up to 6 antibiotics simultaneously. These results provided evidence that the presence of Staphylococcus species in dairy environment are mostly represented by S. equorum and S. saprophyticus, and illustrate that carrying antimicrobial resistance genes has become reasonably widespread in cheese and dairy environment.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6699
Versão do Editor: doi:10.1016/j.ijfoodmicro.2011.02.008
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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