Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6688
Título: Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels
Autor: Silva, Joana
Carvalho, Ana Sofia
Votorino, Rui
Domingues, Pedro
Teixeira, Paula
Gibbs, paul
Palavras-chave: Lactobacillus bulgaricus
Protein extraction
2-DE electrophoresis
Staining
Sensitivity
Data: 2004
Editora: Bentham Science Publishers Ltd.
Citação: "Protein and peptide letters" . ISSN 0929-866511:2 (2004) 189-194
Resumo: Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.
URI: http://hdl.handle.net/10400.14/6688
Versão do Editor: http://www.benthamscience.com/ppl/
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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