Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6669
Título: Rapid spectrophotometric determination of nitrates and nitrites in marine aqueous culture media
Autor: Carvalho, A. P.
Meireles, L.A.
Malcata, F.X.
Palavras-chave: Enriched sea water microalgae broth
Nitrate quantification
Nitrite quantification
Data: 1998
Editora: EDP Sciences, Wiley-VCH
Citação: CARVALHO, A. P.; MEIRELES, L. A.; MALCATA, F.X. - Rapid spectrophotometric determination of nitrates and nitrites in marine aqueous culture media. Analusis. ISSN 0365-4877. Vol. 26 (1998), p. 347-351
Resumo: The spectrophotometric determination of nitrate in sea water broths for cultivation of, say, microalgae is complicated by the frequent presence of nitrite. Two methods - sulphamic/perchloric acid method (also known as Cawse method) and sulphamic acid method - both claimed to be able to eliminate nitrite interference, were tested using a set of standards, but statistical treatment of the results proved their limitations in nitrate quantification. An improved method, based on former published methods for quantification of nitrite and coupled determination of nitrate and nitrite, was designed and tested. This improved method was compared with the reference method (based on use of a cadmium column) using several standards and biological samples of two culture media for microalgae, in different phases of their growth curve. The results thus obtained have demonstrated that there is no statistically significant difference between them at the 5% level. The precision of the method was tested by repeating determinations with three sets of standard mixtures containing nitrate and nitrite. The method proposed has advantages over conventional methods in reduced time of analysis, as well as high precision and accuracy, so it may be a good alternative for determination of nitrite and nitrate in marine aqueous media.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6669
Versão do Editor: http://analusis.edpsciences.org/index.php?option=com_article&access=standard&Itemid=129&url=/articles/analusis/pdf/1998/09/s110998.pdf
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
Rapid spectrophotometric determination of nitrates and nitrites in marine aqueous culture media.pdf63,72 kBAdobe PDFVer/Abrir

FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.