Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6580
Título: Enzymatic determination of L(+) lactic and L(-) malic acids in wines by flow-injection spectrophotometry
Autor: Lima, José L. F. C.
Lopes, Teresa I. M. S.
Rangel, António O. S. S.
Palavras-chave: Sequential Flow injection
L(+) lactic acid
L(-) malic acid
Spectrophotometric enzymatic determination
Wines
Data: 1998
Editora: Elsevier
Citação: LIMA, José L. F. C.; LOPES, Teresa I. M. S. ; RANGEL António O.S.S. - Enzymatic determination of L(+) lactic and L(-) malic acids in wines by flow-injection spectrophotometry. Analytica Chimica Acta. ISSN 0003-2670. Vol. 366 (1998), p. 187-191
Resumo: A flow-injection system for the enzymatic determination of L( ) lactic acid and L(ÿ) malic acid in wines with spectrophotometric detection is described. The samples are dialysed in-line, and the enzymes in solution (malate dehydrogenase and lactate dehydrogenase) are injected as a train of plugs in the acceptor stream of the dialysis unit, yielding two peaks corresponding to the NADH formed for each determination. This methodology enables the determination of both acids with a single detector with a sampling rate of 20 h ÿ1 (0.4±3 g l ÿ1). The results are comparable to those obtained by the reference procedure, the repeatability is better than 5% (rsd), with low enzyme consumption (1.3 ml of suspension per sample).
Peer review: yes
URI: http://hdl.handle.net/10400.14/6580
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0003267098001068
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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